Influence of Organic-Phosphates on 3-Phosphoglycerate Dependent O_2 Evolution in C_3 and C_4 Mesophyll Chloroplasts
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概要
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In C_4 plants phosphoenolpyruvate (PEP) of the C_4 cycle may be transported on a chloroplast transporter which also transports 3-phosphoglycerate (3-PGA) and triose-phosphates. In C_3 plants PEP is not considered to be effectively transported on the chloroplast phosphate translocator. The influences of certain organic phosphates, having a similar structure to either PEP or triose-phosphates, on 3-PGA dependent O_2 evolution by C_4 (Digitaria sanquinalis L. Scop.) and C_3 (Hordeum vulgare L.) mesophyll chloroplasts were investigated. In the C_4 mesophyll chloroplasts phosphoglycolate was a competitive inhibitor (K_i=2.1 mM) of 3-PGA dependent O_2 evolution, and was as effective as previously reported for PEP. 2-Phosphoglycerate was also a competitive inhibitor (K_i=8.6 mM) of O_2 evolution in the C_4 mesophyll chloroplasts with 3-PGA as substrate, while phospholactate was a weak inhibitor and glyphosate had no effect. Neither PEP, phosphoglycolate nor 2-phosphoglycerate were effective inhibitors of 3-PGA dependent O_2 evolution in the C_3 chloroplasts. Phosphohydroxypyruvate was a competitive inhibitor of 3-PGA dependent O_2 evolution in both chloroplast types. The selectivity in inhibition of O_2 evolution with 3-PGA as substrate suggests that the C_4 mesophyll chloroplasts can recognize certain organic phosphates with the phosphate in the C-2 or C-3 position but that the C_3 mesophyll chloroplasts can only effectively recognize certain organic phosphates with the phosphate in the C-3 position. The results also support the view that 3-PGA and PEP are transported on the same phosphate translocator in C_4 mesophyll chloroplasts.
- 日本植物生理学会の論文
著者
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Edwards Gerald
Department Of Botany Washington State University
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Wessinger M
Washington State Univ. Wa Usa
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Rumpho M
Texas A & M Univ. Tx Usa
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Rumpho Mary
Department of Botany, Washington State University
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Wessinger Mary
Department of Botany, Washington State University
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Rumpho Mary
Department Of Biochemistry Microbiology And Molecular Biology
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