Characterization of Cu and Zn Resistant Nicotiana plumbaginifolia Suspension Cultures

概要

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The mechanisms of Cu and Zn resistance in cultured Nicotiana plumbaginifolia cells were investigated. When crude extracts of both the Cu and Zn resistant cells were passed through Sephadex G-75 gel filtration columns, two metal containing peaks were identified. In each case the Cu or Zn was associated with a high molecular weight fraction (ca. 70,000) and a low molecular weight fraction (less than 3,000) with the majority being associated with the low molecular weight fraction. The low molecular weight fraction in particular increased upon exposure of the cells to the metals. Both the Cu and Zn resistant cells accumulated higher levels of citrate and malate than the wild type cells in the presence of the metals. The addition of citrate or malate, but not fumarate or succinate to the culture medium prevented Cu or Zn toxicity to wild type cells. These results suggest that the N. plumbaginifolia cells selected as resistant to Cu or Zn are resistant, at least in part, because of their ability to accumulate the chelators, citrate and malate, in the presence of the metals.
日本植物生理学会の論文