The Ca^<2+>-Induced Phase Transformation in Soybean Root Microsomal Membrans is a Consequence of Phospholipase Activation

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概要

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• Microsomes from soybean (Glycine max L.) roots contain a Ca^<2+>-activated phospholipase D which hydrolyzes phosphatidylethanolamine and phosphatidylcholine to phosphatidic acid. The phosphatidic acid binds Ca^<2+> in the medium and undergoes a liquid crystal to gel phase transformation (measured by wide-angle x-ray diffraction). The gel phase formation in the microsomes half-saturates at 1 to 10 mM Ca^<2+>. The upper limit for the transition temperature for the microsomes is -10 to 10℃ in the native state. After treatment with Ca^<2+>, the transition temperature in-creases to 35 to 50℃. Under the same experimental conditions, incubation with 10 mM Ca^<2+> at 4℃ causes an increase in phosphatidic acid content from 8 mole % to 49% with a concomitant decrease in phosphatidylethanolamine and phosphatidylcholine from about 22% and 41%, respectively, to 14% each. These results suggest that Ca^<2+> effects in systems where Ca^<2+> plays a multifunctional role be interpreted with caution.

• 日本植物生理学会の論文

著者

• Lew R

  York Univ. Ontario Can

• Caffrey Martin

  Section of Biochemistry, Molecular and Cell Biology, Clark Hall, Cornell University

• Lew Roger

  Section of Plant Biology, Division of Biological Sciences, Plant Science Building,. Cornell Universi

• Caffrey Martin

  Section Of Biochemistry Molecular And Cell Biology Clark Hall Cornell University

関連論文

• The Ca^-Induced Phase Transformation in Soybean Root Microsomal Membrans is a Consequence of Phospholipase Activation

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