Protein Synthesis Linked to Respiration and Phosphorylation in Intact Euglena Mitochondria

概要

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Highly purified condensed mitochondria obtained from bleached mutant W_<10>BSmL of Euglena gracilis Klebs var bacillaris Cori incorporate [<35>^S]methionine into protein when fortified with malate, ADP, Mg^<2+>, phosphate and a sucrose osmoticum. Twenty to twenty-five polypeptide bands were found to be labeled in organello wheh the labeled protein was subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis. Methionine incorporation, but not respiration or oxidative phosphorylation, was blocked by chloramphehicol and other 70S ribosomal translation inhibitors but cycloheximide and ribonuclease were without effect. Inhibitors of electron transport and uncouplers of oxidative phosphorylation were excellent inhibitors of protein syhthesis. Thus, these mitochondrial preparations carry out protein synthesis in organello that is linked to respiration and oxidative phosphorylation.
日本植物生理学会の論文