Acifluorfen and Oxidized Ubiquinone in Soybean Mitochondria

概要

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Intact mitochondria from soybean [Glycine mex (L.) Merrill cv Braggl seedlings were incubated with acifluorfen {sodium-5-[2-chloro-4-(trifluoromethyl)phenoxyl-2-nitrobenzoate}. Respiration, measured as O_2 consumption, was inhibited by acifluorfen with a 50% inhibition occurring with 10μM of the herbicide. The inhibition was unaffected by illumination; moreover, prior illumination by either UV or visible light did not alter the inhibition capacity ofacifluorfen. Further examination of the locus of acifluorfen inhibition at the electron transport chain level showed no inhibition in Cyt c oxidation capacity as well as no effect on NADH dehydrogenase, succinate dehydroge-nase, malate dehydrogenase and isocitrate dehydrogenase. It was observed, however, that in mitochondria incubated for 10 min with 0.2 ,μM acifluorfen, ubiquinone existed predominantly in the oxidized state. We conclude that at the mitochondrial level, a possible major site of action of acifluorfen is either a direct or indirect modulation of the redox potential of ubiquinone.
日本植物生理学会の論文