O-Benzylhydroxylamine An Inhibitor of Phenylpropanoid Metabolism in Plants

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概要

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• O-Benzylhydroxylamine (OBHA) is a potent inhibitor of phenylalanine ammonialyase (PAL, EC 4.3.1.5) and phenylpropanoid metabolism as evidenced by its effects on three plant species [soybean (Glycine max (L.) Merr.), buckwheat (Fagopyrum esculentum Moench.), and mung bean (Vigna radiata L.)]. When supplied to roots, OBHA (10^<-5> M) did not significantly inhibit light- or dark-growth of soybean seedlings, but reduced (25%) soluble hydroxyphenolic compound accumulation in light-grown axes. Higher concentrations (5×10^<-5> M) of OBHA caused reductions (25%) in axis fresh weight of light-grown seedlings (72 h), but did not lower axis weight of dark-grown seedlings. Anthocyanin accumulation in hypocotyls of intact mung bean seedlings was reduced by 25% after 3 days light growth after treatment with OBHA (10^<-5> M) via root feeding. Anthocyanin content of excised, etiolated buckwheat hypocotyls floated on solutions of OBHA (10^<-5> M) and incubated in the light for 24 h was reduced by 40%. L-Phenylalanine and t-cinnamic acid, intermediates of phenylpropanoid metabolism, were able to partially reverse this inhibition in buckwheat. Extractable PAL activity (specific activity basis) in soybean axes was substantially reduced (20% in dark, 40% in light) as early as 24 h after root feeding with OBHA (10^<-5> M). Reduction of PAL activity (specific activity or per axis basis) by OBHA compared to control levels, continued throughout a time course of 96 h. Kinetic studies on soybean PAL revealed a K_m of 1.1 mM for L-phenylalanine and an apparent K_i of 3.5 μM for OBHA.

• 日本植物生理学会の論文

著者

• Hoagland Robert

  Usda Ars Southern Weed Science Laboratory

• Hoagland Robert

  USDA, ARS, Southern Weed Science Laboratory

関連論文

• O-Benzylhydroxylamine An Inhibitor of Phenylpropanoid Metabolism in Plants
• Influence of Glyphosate on Nitrate Reductase Activity in Soybean (Glycine max)

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