High Performance Liquid Chromatography of Molecular Species from Free Sterols and Sterylglycosides Isolated from Oat Leaves and Seeds

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概要

• 論文の詳細を見る

• Free sterols and sterylglycosides (SG) from oat leaves and seeds were isolated by conventional thin layer chromatography (TLC) and subjected to high performance liquid chromatography (HPLC) for resolution of molecular species. Acylsterylglycosides, isolated by TLC, were converted to SG by mild alkaline hydrolysis and determined as SG. Sterols and SG were injected onto the column without any chemical treatment and the separated species were detected at 200 nm. The separation of SG-species follows exactly the separation of free sterols. Though gas liquid chromatography still is the method of choice, advantages of HPLC is to analyse directly the SG-species without hydrolysis and derivatization as compared to GLC. After TLC the sterol- and the SG-fraction are injected directly onto the column. This is extremely important for labile sterylglycosides or sterols, as demonstrated for the avenasterols.

• 日本植物生理学会の論文

著者

• Eichenberger W

  Univ. Bern Che

• Kesselmeier Jurgen

  Botanical Institute, University of Cologne

• Eichenberger Waldemar

  Biochemical Institute, University of Berne

• Urban Birgit

  Biochemical Institute, University of Berne

• Urban Birgit

  Biochemical Institute University Of Berne

• Kesselmeier J

  Max Planck Inst. Chemistry Mainz Deu

関連論文

• High Performance Liquid Chromatography of Molecular Species from Free Sterols and Sterylglycosides Isolated from Oat Leaves and Seeds
• Changes of the Galactolipid Composition during Illumination of Isolated Oat Etioplasts

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