Reversed-Phase High-Performance Liquid Chromatography of Prenylquinones in Green Leaves Using an Electrochemical Detector

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概要

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• Ubiquinone-9, -10, plastoquinone-A, -B, -C, phylloquinone and a-tocopherolquinone in spinach leaf extract were separated and determined by reversed-phase high-performance liquid chromatography using an electrochemical detector. These prenyl-quinones were eluted with a mixture of ethanol and methanol containing 50 mM NaClO_4 and 2 mM HClO_4 from an octadec.yl silica column. The electrochemical detector could selectively detect the quinones in the eluate, and enabled to determine even the minor quinones such as PQ:-B and PQ-C which had not been evaluated by HPLC with an optical detector. The method is simple and sensitive to the degree that amounts of prenylquinones could be determined as low as 0.1 nmol.

• 日本植物生理学会の論文

著者

• Okayama S

  Kyushu Univ. Fukuoka Jpn

• Okayama Shigeki

  Biological Laboratory College Of General Education Kyushu University

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• Reversed-Phase High-Performance Liquid Chromatography of Prenylquinones in Green Leaves Using an Electrochemical Detector
• Simultaneous Determination of Oxidized and Reduced Forms of Plastoquinone A in Spinach Chloroplasts by High-Performance Liquid Chromatography with an Electrochemical Detector
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