Thermosensitive Export of Amylases in the Yeast Schwanniomyces alluvius

概要

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Cultures of Schwanniomyces alluvius were grown aerobically at 30℃ in a phosphate-buffered defined medium containing soluble starch as sole carbon source. Extracellular amylolytic activity, minimal when cells were most actively dividing, increased dramatically at the end of exponential growth and became maximal during stationary phase. When cultures were grown at 37℃, no increase in extracellular amylolytic activity was detectable. This lack was only partly ascribable to thermal inactivation of the amylases. When cultures growing at 30℃ were shifted to 37℃, excretion was arrested. Cultures growing at the restrictive temperature could be made to excrete full amylolytic activity at the permissive temperature, only if the shift was made before the end of exponential growth.
日本植物生理学会の論文