Photosynthesis in Cell Suspension Culture of a C_4 Plant, Gisekia pharnaceoides L.

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Photomixotrophic cell suspension culture was established from the leaf derived callus cells of Gisekia pharnaceoides L., a C_4 dicotyledonous weed. The late log phase cells possessed shade-type characters such as low chlorophyll a/b ratio, less pronounced O_2 evolution and CO_2 fixation, saturation of photosynthetic CO_2 fixation at low intensity. The chloroplasts from these cells contained granal stacking with high degree of a very few grana which are characterized by their wide and high degree of stackings. The predominant labelling of 3-phosphoglyceric acid and sugar phosphates (40% of the total ^<14>C incorporated) during 5 s exposure to ^<14>CO_2 in light and subsequent decrease in percentage of ^<14>C in these compounds with increase in exposure time indicated the operation of the C_3 pathway in these cells. The simultaneous synthesis of malate (23% of the total ^<14>C incorporated) is related to the much pronounced glycolytic and tricarboxylic acid cycle activities in these cells. The initial proliferation of calli mainly from the zones of vascular supplies in the leaf, high starch content of the cells, presence of large starch grains in all the chloroplasts, activities of Calvin cycle enzymes, heavy labelling of C_3 type intermediates and less labelling of aspartate as early photosynthates and rapid accumulation of radioactivity into starch during ^<14>CO_2 assimilation indicated that most of the cells in photomixotrophic culture were derived from bundle sheath cells or the leaf cells of Gisekia change their function under culture conditions.
日本植物生理学会の論文

Photosynthesis in Cell Suspension Culture of a C_4 Plant, Gisekia pharnaceoides L.

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