Effects of Metabolic Inhibitors on Anthocyanin Accumulation in Petals of Rosa hybrida Hort. cv. Ehigasa

概要

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Anthocyanin accumulation of petal disks of Rosa hybrida Hort. cv. Ehigasa was strongly inhibited by 2,4-dinitrophenol (2,4-DNP), suggesting that this process requires AtP. The inhibition could not be reversed by kinetin or naringenin, but could be when both were given together. This implies that 2,4-DNP exerted its effect on the step after flavanone formation. When kinetin was added simultaneously with naringenin to the medium, naringenin was considered to have been converted into anthocyanin in cytosol and transported into the vacuoles with the aid of kinetin. When naringenin was added to the medium, the disks contained a larger amount of naringenin 7-glucoside than when it was not added. Naringenin is thought to have been metabolized to glucoside and/or converted into anthocyanin in the cells. Anthocyanin accumulation in the petal disks was not inhibited by N,N'-dicyclohexylcarbodiimide (DCCD), whereas 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDAC) caused strong inhibition which could not be reversed by adding naringenin to the medium. As these reactions seem to be similar way to those observed for tonoplast ATPase with the same inhibitors, anthocyanin permeation into vacuoles may be mediated by tonoplast ATPase, which might be stimulated by UV irradiation.
日本植物生理学会の論文