Inhibition of Photosynthetic Oxygen Evolution in Thylakoids by Cyanide
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概要
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Cyanide inhibition of oxygen evolution from isolated chloroplasts was accomplished by altering the thylakoid membrane properties (in addition to charge-screening) by high concentrations of Mg^<2+>. The divalent cation facilitated the approach of the cyanide ligand (pK=9.4) to the water-splitting site. The addition of the divalent cation ionophore, A23187, in the presence of Mg^<2+> enhanced the expression of cyanide inhibition of photosystem II-mediated O_2-evolution. Furthermore, electron transport partial reactions allowed the identification of the site of cyanide action as being between the site of Tris-inhibition of electron transport and the site of diphenylcarbazide donation on the oxidizing side of PS II.