Temperature-Induced Changes of Variable Fluorescence-Yield in Intact Leaves
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概要
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Variable fluorescence (F_v) of intact leaves was measured when the temperature was lowered at a rate of 1-2℃ per min, from 20℃ to -20℃. The quantum flux density of the exciting light was 1-2μE m^<-2> sec^<-1> in order to sensitize F_0 only at 20℃. The fluorescence yield decreased rapidly at the freezing point of the leaf and upon further cooling the fluorescence yield increased again. F_m was obtained a few degrees below the freezing point. Repeated freeze-thaw cycles caused successively increased damage to the thylakoid membranes on either the oxidizing or the reducing side of photosystem II. An eventual loss of F_v over F_0 was typical for damage on the water splitting side of photosystem II, whereas damage after the primary electron acceptor Q of photosystem II was characterized by an invariable fluorescence yield at F_m over the temperature range examined.
- 一般社団法人日本植物生理学会の論文
著者
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Oquist Gunnar
Department of Plant hysiology, University of Umea
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Sundbom Erik
Department of Plant Physiology, University of Umea
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- Temperature-Induced Changes of Variable Fluorescence-Yield in Intact Leaves