Inhibition of Membrane-Bound Adenosine Triphosphatase Activity from Nicotiana tabacum L. Leaves with Alkyltrimethylammonium Bromide

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概要

• 論文の詳細を見る

• Cetyltrimethylammonium bromide (CTAB), a cationic detergent, more effectively inhibited the activity of membrane-bound epidermal adenosine triphosphatase (ATPase) of tobacco (Nicotiana tabacum L. cv. Samsun) leaves than anionic or nonionic detergents. The inhibition of ATPase activity was highly dependent on the length of the alkyl chain of alkyltrimethylammonium: CTAB > dodecyltrimethylammonium bromide > n-octyltri-methylammonium bromide ≒ trimethylammonium chloride ≒ cetyl bromide, compared at 10^<-4> M. The last three derivatives hardly inhibited the activity. CTAB inhibition was equivalent to that due to other cationic detergents, cetylpyridinium bromide and cetyl amine, but less than that by gramicidin S and tyrocidine and stronger than that by N,N'-dicyclohexylcarbodiimide and vanadate. These results show that a certain length of the alkyl chain (C_n>12) and the combination of both hydrophobic and charged groups of a detergent moiety are indispensable for inhibiting the membrane-bound epidermal ATPase activity.

• 日本植物生理学会の論文

著者

• Kasamo Kunihiro

  Institute 0f Applied Biochemistry, University of Tsukuba

• Kasamo Kunihiro

  Institute for Plant Virus Research, Tsukuba Science City

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• Inhibition of Membrane-Bound Adenosine Triphosphatase Activity from Nicotiana tabacum L. Leaves with Alkyltrimethylammonium Bromide
• Gramicidin S : A Potent Inhibitor of Membrane-bound Epidermal Adenosine Triphosphatase from Nicotiana tabacum L. Leaves
• The role of the epidermis in kinetin-induced retardation of chlorophyll degradation in tobacco leaf discs during senescence

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