Free Radical-mediated Formation of Ethylene from 1-Aminocyclopropane-1-carboxylic Acid : A Spin-trap Study

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概要

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• The ability of free radicals to convert 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene under strictly chemical conditions has been investigated using the aerobic xanthine/xanthine oxidase reaction and the Fenton reaction. Ethylene is formed when 1 m_M ACC is added to either of these reactions. Ethylene production by the xanthine/ xanthine oxidase system can be stimulated by H_2O_2 and inhibited by both catalase and superoxide dismutase, suggesting that the hydroxyl radical (OH・) formed by the Haber-Weiss reaction is reacting with ACC to form ethylene. Ethylene production from ACC by the Fenton reagent, which also produces OH・, shows a strong dependence upon H_2O_2. Involvement of the OH・ radical was confirmed by spin-trap studies using 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). Only the hydroxyl adduct of DMPO was detectable in both the xanthine/xanthine oxidase reaction and the Fenton reaction. When ACC was added to the Fenton reaction, an additional adduct of DMPO was detectable, which, on the basis of its hyperfine splitting constants, can be tentatively identified as the DMPO adduct of a carbon-centered free radical. The data are consistent with the view that formation of ethylene from ACC entails attack by OH・ and the resultant formation of a carbon-centered radical, possibly of ACC. The chemical conversion of ACC to ethylene is less efficient than that characteristic of senescing tissues, in which the reaction is enzymatically mediated.

• 日本植物生理学会の論文

著者

• Thompson J.e.

  Department Of Biology University Of Walerloo

• Legge R.L.

  Department of Biology, University of Walerloo

• Baker J.E.

  Plant Hormone Laboratory, ARS, Plant Physiology Institute, United Stales Department of Agriculture

• Baker J.e.

  Plant Hormone Laboratory Ars Plant Physiology Institute

• Baker J.e.

  Plant Hormone Laboratory Ars Plant Physiology Institute United Stales Department Of Agriculture

• Legge R.l.

  Department Of Biology University Of Walerloo

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• Free Radical-mediated Formation of Ethylene from 1-Aminocyclopropane-1-carboxylic Acid : A Spin-trap Study
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