Messenger RNA in Embryonic Axes of Dormant and Non-Dormant Seeds of Hazel (Corylus avellana L.)

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GA_3 and cotyledon excision (which break dormancy) change the size distribution of RNA containing polyadenylic acid [poly(A)^+RNA] synthesised at 24 hr in the embryonic axes of hazel (Corylus avellana L.) seeds. This difference precedes germination by at least five days. GA_3 induces a similar size change in excised axes in liquid culture. Poly(A)^+RNA from dormant and non-dormant seeds support in vitro translation into a large number of polypeptides, most of which are present in both treatments. GA_3 represses the synthesis of some peptides and promotes the synthesis of others. The change in size and encoded peptides of poly(A)^+RNA is the first direct evidence consistent with the suggestion that dormancy breaking may be associated with the transcription of novel mRNAs specific for germination. After two-dimensional electrophoresis, the pattern of polypeptides labelled in vivo is different from the pattern of peptides synthesised in vitro, suggesting that there is strong translational control in hazel axes. The poly(A) in the newly synthesised poly(A)^+RNA from axes of dormant and non-dormant seeds are of similar size. A greater level of messenger activity is detectable in RNA lacking poly(A) [poly(A)^-RNA] than in poly(A)^+ RNA in imbibed dormant and non-dormant axes. Poly(A)^+mRNA and poly(A)^-mRNA are present in axes of dry seeds, the former declining markedly during storage.
日本植物生理学会の論文