Chloramphenicol Induced Changes in Some Respiratory Characteristics of Potato Tuber Callus

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Chloramphenicol (CAP), an inhibitor of the mitochondrial protein synthesis inhibits callus induction and subsequent growth of potato tuber tissue discs. Tissue respiration increase did not occur in the presence of CAP. Both with and without CAP the initially CN^--sensitive tissue becomes totally CN^--resistant in 1-2 weeks. CAP blocks the development of mitcohondrial cytochrome oxidase. A gradual decrease in the activities of cytochrome oxidase and of cytochrome pathway-mediated mitochondrial respiration is found in CAP-tissue. The mitochondrial alternative pathway which is absent in mitochondria from frdshly sliced tissue develops during incubation both in the absence and presence of CAP. The alternative pathway is only operative in uninhibited state III respiration in mitochondria from CAP-tissue. Cycloheximide, an inhibitor of the cytoplasmic protein synthesis inhibits the developments of the alternative pathway and of the cytochrome pathway. Alcohol dehydrogenase activity increases tenfold in the tissue during two weeks of incubation on media with and without CAP. Alcohol production in the tissue did not take place in the controls nor in the CAP-treated tissue.
日本植物生理学会の論文