Phase Transition Temperatures Determined for Chloroplast Thylakoid Membranes and for Liposomes Prepared from Charged Lipids Extracted from Thylakoid Membranes of Higher Plants

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Lipid phase separation temperatures of intact thylakoid membranes from a number of chilling sensitive plants were measured using chlorophyll a as the intrinsic fluorescent probe. The phospho- and sulfolipids were extracted from the thylakoid lamellae of these plants and purified by silicic acid column and thin layer chromatographies. These separated lipids were eluted and recombined to give a total charged anionic thylakoid lipid fraction that was used to prepare liposomes containing purified chlorophyll a as the fluorescent probe. The phase separation temperatures of these liposomes were compared to phase separation temperatures in intact thylakoid membranes isolated from the same plants. The chilling-sensitive plants-corn, pepper, tomato and water hyacinth - showed phase separation temperatures ranging from 9 to 19℃ for both the liposomes and the thylakoid membranes. In addition, low temperature phase separations were seen from -21 to -27℃. Mimulus, which is not as chilling sensitive as the former plants, had a phase separation temperature near 0 to 2.5℃ and at -27℃. In general, there was a good agreement between the phase separation temperatures of intact thylakoids and the purified anionic lipid fraction extracted from these thylakoids. Similar results were obtained using either trans-parinaric acid or chlorophyll a as the fluorescent probe in liposomes made from anionic thylakoid lipids or in liposomes prepared from pure dimyristoyl phosphatidyl choline, distearoyl phosphatidyl choline, or mixtures of equal amounts of these phospholipids.
日本植物生理学会の論文

Phase Transition Temperatures Determined for Chloroplast Thylakoid Membranes and for Liposomes Prepared from Charged Lipids Extracted from Thylakoid Membranes of Higher Plants

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