Isozymes of Glucose-6-phosphate Dehydrogenase and NAD^+-malate Dehydrogenase in Shoot-forming Foliar Discs of Tobacco

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概要

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• Discrete pale, meristematic, shoot-forming zones (SF) and green, relatively non-dividing, non-shoot-forming zones (NSF) of cells were obtained from leaf discs of tobacco cultured for 12 days on a shoot-forming medium. Higher chlorophyll and starch content, increased rates of O_2 evolution and CO_2 fixation in light, and increased activities of amylases and chloroplastic enzymes such as ribulose 1,5-bisphosphate carboxylase and NADP^+-linked glyceraldehyde-3-phosphate dehydrogenase (G3PDH) were characteristic of the cells constituting NSF. On the other hand, active participation of sucrose hydrolysis, dark-mediated CO_2 incorporation, an oxidative pentose phosphate pathway, glycolysis and mitochondrial complements in shoot formation were evident from the significantly high activities of phosphoenolpyruvate carboxylase, invertase, glucose-6-phosphate dehydrogenase (G6PDH), NAD^+-G3PDH and NAD^+-linked malate dehydrogenase (NAD^+-MDH) respectively, in SF cells. Detection of activity of the enzymes by staining on polyacrylamide gels disclosed synthesis of additional isoenzyme(s) of G6PDH, NAD^+-MDH and peroxidase in shoot initiation sites. The much pronounced activity and isozyme groups of G6PDH and NAD^+-MDH in the photosynthetically incompetent shoot-forming cells, are considered to increase the carbon budget of the differentiating cells through non-autotrohpic CO_2 fixation and to supplement reducing power (NADPH) for the organogenetic process which requires much energy. The changes in isozymes of these enzymes, as in the isoperoxidase system, probably can serve as useful markers of the differentiation process.

• 日本植物生理学会の論文

著者

• Seeni S

  Department Of Plant Physiology School Of Biological Sciences Madurai Kamaraj University

• Seeni S

  Department Of Plant Sciences School Of Biological Sciences Madurai Kamaraj University

• Gnanan A

  Department of Plant Sciences, School of Biological Sciences, Madurai Kamaraj University

• Gnanan A

  Department Of Plant Sciences School Of Biological Sciences Madurai Kamaraj University

関連論文

• Relationship between Chlorophyll Concentration and Photosynthestic Potential in Callus Cells
• Isozymes of Glucose-6-phosphate Dehydrogenase and NAD^+-malate Dehydrogenase in Shoot-forming Foliar Discs of Tobacco

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