Purification and Properties of the Diamine Oxidase from Vicia faba Leaves

概要

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Diamine oxidase (EC 1.4.3.6) from the leaves of Vicia faba was purified to homogeneity by polyacrylamide gel electrophoresis. The molecular weight estimated by Sephadex G-200 gel filtration was about 126,000. Sodium dodecyl sulfate gel electrophoresis gave a single band at the molecular weight of 74,000. The isoelectric point was at pH 7.2. The enzyme contained two copper atoms per mole of enzyme. Inhibition with phenylhydrazine showed that the Vicia enzyme contains one mole of the carbonyl group per mole of the enzyme. The amino acid composition of the enzyme also is described.
日本植物生理学会の論文