Induction, selection and isolation of auxin hetetotrophic and auxin-resistant mutants from cultured crown gall cells irradiated with gamma rays

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概要

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• Cultured crown gall cells were irradiated with gamma rays to induce mutation in indoleacetic acid biosynthesis. The irradiated cells were plated on a selection medium which contained auxin. Mutant cells adapted to selection media were characterized as auxin-heterotrophic and auxin-resistant cell lines. The auxin-heterotrophic mutants contained little auxin, whereas the auxin-resistant and -autotrophic mutants contained large amounts of auxin even when cultured with 0.3 ppm of 2,4-dichlorophenoxyacetic acid. Each mutant cell line contained as much octopine as its parental cells. The mutation rate was calculated as in the order of 10^<-8>.

• 日本植物生理学会の論文

著者

• Atsumi Shigeaki

  Department Of Bioldgy College Of General Education University Of Tokyo Komaba

関連論文

• The relationship between auxin concentration, auxin protection and auxin destruction in crown gall cells cultured in vitro
• Studies on the production of indole-3-acetic acid with auxin-heterotrophic mutants derived from cultured crown gall cells
• Induction, selection and isolation of auxin hetetotrophic and auxin-resistant mutants from cultured crown gall cells irradiated with gamma rays
• Relation between auxin autotrophy and tryptophan content in sunflower crown gall cells in culture
• Examination of the pronounced increase n auxin content of senescent leaves

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