Characterization of new strains of nonphotosynthetic mutants of Chlamydomonas reinhardtii II. : Quinones and cytochromes b-559, b-563 and c-553 in twelve mutants having impaired Photosystem II function

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Twelve new strains of nonphotosynthetic mutants of Chlamydomonas reinhardtii having impaired functioning of Photosystem II were studied with respect to their quinone and chloroplastic cytochrome contents and to various photooxidation reactions of cytochromes b-559 and c-553. The quinones were analyzed by chromatography, cytochromes b-563 and c-553 were measured spectrophotometrically after solubilization by Triton X-100, and cytochrome b-559 was studied by means of low-temperature difference spectra. None of these mutants showed a great deficiency of plastoquinone A, ubiquinone Q9, cytochrome b-563 or cytochrome c-553. But all lacked an ascorbate-reducible pool of cytochrome b-559 photooxidizable at 77゜K. In spite of this deficiency, five mutants (Fl 18, Fl 29, Fl 47, Fl 50, Fl 59) showed an appreciable photooxidation of cytochrome b-559 in the presence of FCCP at room temperature. The other strains performed only weak cytochrome b-559 photooxidation in the presence of FCCP, DCMU and DBMIB or p-benzoquinone (Fl 39, Fl 42, Fl 52, Fl 54, Fl 57, Fl 60) ; in the mutant Fl 33, no cytochrome photooxidation was observed. These results pointed out that the pool of ascorbate-reducible cytochrome b-559 photooxidizable at 77゜K is different from the pool photooxidizable in the presence of FCCP at room temperature.
日本植物生理学会の論文