Incorporation of ^3H-uridine and ^3H-leucine during early embryogenesis of rice and barley in caryopsis culture

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概要

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• Incorporation of ^3H-uridine and ^3H-leucine during early embryogenesis in rice and barley was examined by culturing caryopses on White's medium containing these isotopes. Grain counts showed that no detectable amount of ^3H-uridine was incorporated during the early and middle periods of the globular embyo in both plants. ^3H-Uridine was detected in an embryo consisting of more than 100 cells. ^3H-Leucine, however, was incorporated from the beginning of embryogenesis. These results suggest that during early embryonic development in these plants, as in animal embryogenesis, RNA synthesis is limited and the proteins will be synthesized by using RNAs stored in the egg cell. The 100-cell stage is considered a critical point in early embryonic development, because at this stage cereal embryos start exponential and dual rhythmic growth as well as uridine incorporation.

• 日本植物生理学会の論文

著者

• Nagato Yasuo

  Laboratory Of Plant Breeding Faculty Of Agriculture University Of Tokyo

関連論文

• Incorporation of ^3H-uridine and ^3H-leucine during early embryogenesis of rice and barley in caryopsis culture

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