Effect of abscisic acid on membrane-bound epidermal ATPase from tobacco leaves

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概要

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• Membrane-bound Mg^<++>-activated ATPase activity in epidermal strips from tobacco leaves (Nicotiana tabacum L. Samsun NN) was stimulated by abscisic acid (ABA) when the strips were floated on ABA solution in light or in darkness. The optimum ABA concentrations in light and in darkness were 10^<-5> _M and 10^<-6> _M, respectively. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) and N,N'-dicyclohexylcarbodiimide (DCCD) completely blocked the basal level membrane-bound epidermal ATPase activity. ABA-induced membrane-bound epidermal ATPase activity was completely inhibited by CCCP, but only partly by DCCD. H^+-influx into epidermal strips on a solution in light was lower than that in darkness. ABA stimulated H^+-influx into epidermal strips in light and in darkness. CCCP suppressed basal level H^+-influx, whereas DCCD did not. CCCP also suppressed ABA-induced H^+-influx, whereas DCCD did not. Interaction between H^+-influx and membrane-bound epidermal ATPase activity is discussed.

• 日本植物生理学会の論文

著者

• Kasano Kunihiro

  Institute For Plant Virus Research Tsukuba Science City

関連論文

• Effect of abscisic acid on membrane-bound epidermal ATPase from tobacco leaves
• Characterization of membrane-bound Mg^-activated ATPase isolated from the lower epidermis of tobacco leaves

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