SYNCHRONIZATION OF BACTERIAL CULTURE BY PREINCUBATION OF CELLS AT HIGH CELL DENSITY

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概要

• 論文の詳細を見る

• The growth of Escherichia coli strain B in a liquid medium was found to cease at a cell density of 5 × 10^9 cells per ml. (This critical concentration is designated as the maximum or M-concentration.) Even cells harvested from the logarithmic growth phase could not divide at this or higher cell densities. Investigations on the metabolic activities of such cultures, however, showed that the synthesis of cellular protein and nucleic acid was taking place under such circumstances, showing that only some process (or processes) particularly related to cell division was suppressed at the critical cell concentration in question. This finding led us to devise a new method of synchronization of E. coli: cells harvested from a logarithmic phase-culture were preincubated of the critical concentration of 5 × 10^9 ceils per ml for 45 minutes, and then diluted 100 times with fresh medium. This led to synchronization of cell division, as shown by a stepwise multiplication in cell number.

• 日本植物生理学会の論文

著者

• Yanagita Tomomichi

  Institure Of Applied Microbiology University Of Tokyo

• KANEKO KENYO

  Institute of Food Microbiology, Chiba University

• Kaneko Kenyo

  Institute Of Food Microbiology Chiba University

• Yanagita T.

  Institure of Applied Microbiology, University of Tokyo

• Yanagita T.

  Institute of Food Microbiology, Chiba University

関連論文

• ORIGIN OF AMINO ACIDS CONSTITUTING CELLULAR PROTEIN IN GERMINATING CONIDIOSPORES OF ASPERGILLUS NIGER
• SYNCHRONIZATION OF BACTERIAL CULTURE BY PREINCUBATION OF CELLS AT HIGH CELL DENSITY
• Dynamic aspects of fungal growth with special reference to enzyme formation (Proceedings of the U.S.Japan seminar on dynamics of microbial populations(特集))

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