MODE OF NUCLEAR DIVISION IN SYNCHRONOUS CULTURES OF CHLORELLA : COMPARISON OF VARIOUS METHODS OF SYNCHRONIZATION

スポンサーリンク

概要

• 論文の詳細を見る

• 1. Chlorella ellipsoidea was grown synchronously using various possible techniques and the mode of nuclear division in each case was followed by staining the nuclei according to FEULGEN. 2. A satisfactory synchrony in respect to nuclear and cellular division was obtained by starting the culture from a homogeneous population of young and small cells and by discontinuing the illumination at the stage which was called the L_3-stage. The starting young cells were invariably mononuclear and the L_3-cells were either dinuclear or tetranuclear. When the L_3-cells were incubated in the dark, they ripened further, and after passing through a tetranuclear stage (referred to as the L_4-stage) divided into four mononuclear daughter cells which have been called the D_n-cells. The most clear-cut and repetitive synchronous culture was obtained when the culture (in the light) was started from the D_n-cells and the illumination was discontinued at the L_3-stage until the fully ripened cells divided into four each of D_n-cells. 3. An apparently "synchronous" culture was also obtained by the method of programmed light-and-dark regimen, in which a random culture is subjected to a regular alternation of light and dark periods of adequate durations. In this case, however, the cells at different stages of culture showed irregular nuclear patterns, and the average "division number" of mother cells was not constant, being subject to change between 4.0 and 4.9.

• 日本植物生理学会の論文

著者

• Tamiya Hiroshi

  The Institute Of Applied Microbiology University Of Tokyo And The Tokugawa Institute For Biological

• Morimura Yuji

  The Institute Of Applied Microbiology University Of Tokyo And The Tokugawa Institute For Biological

• YOKOTA MIZUE

  The Institute of Applied Microbiology, University of Tokyo, and The tokugawa Institute for Biologica

• KUNIEDA REIKO

  The Institute of Applied Microbiology, University of Tokyo, and The tokugawa Institute for Biologica

• Yokota Mizue

  The Institute Of Applied Microbiology University Of Tokyo And The Tokugawa Institute For Biological

• Kunieda Reiko

  The Institute Of Applied Microbiology University Of Tokyo And The Tokugawa Institute For Biological

• Tamiya H.

  The Institute of Applied Microbiology, University of Tokyo, and The tokugawa Institute for Biological Research

関連論文

• MODE OF NUCLEAR DIVISION IN SYNCHRONOUS CULTURES OF CHLORELLA : COMPARISON OF VARIOUS METHODS OF SYNCHRONIZATION

スポンサーリンク