SPORULATION OF THE YEAST, HANSENULA SATURNUS I. EFFECT OF MAGNESIUM AND CALCIUM IONS

概要

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Sporulation in a strain on the wild yeast, Hansenula saturnus, was investigated. The yeast was found to form spores even in distilled water. The sporulation rate (percentage of ascus-bearing individuals) in this case was found to be markedly affected by the cell concentration adopted in the test. The addition of inorganic nutrients to the sporulation medium stimulates sporulation The yeas requires either magnesium or calcium for-growth and sporulation. Higher concentrations of these ions are recolored for sporulation than for growth. In both cases magnesium is effective at more dilute concentrations than calcium. Under the conditions of the experiments, in which the yeast forms a pellicle, the sporulation rate in the pellicle far exceeds that in the sediment. The effects of environmental factors on the sporulation was considered in relation to growth. It was found that, under the conditions of poor growth in the sporulation culture, no exogenous magnesium and calcium are required for sporulation. In such cases, the yeast cells are inferred to have an endogenous stock of magnesium and calcium enough for the sporulation.
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