Effects of kinetin and gamma radiation on the growth and chlorophyll synthesis of isolated radish cotyledons

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Seeds of Raphanus sativus L. were irradiated with 120 and 240 Kr of gamma rays from a ^<137>Cs source. Chlorophyll synthesis and fresh weight increases (an estimate of cell expansion) of isolated cotyledons proved to be radioresistant processes. A lag phase in chlorophyll synthesis, removable by kinetin (10 mg/liter), was observed. Gamma radiation decreased the reactivity to kinetin in the removal of the lag phase, but did not affect the kinetin-stimulated fresh weight increase both in light and dark. Addition of 8-azaguanine to the incubation media reduced the kinetin-stimulated fresh weight in-crease. This inhibition was significantly enhanced by gamma radiation when the cotyledons were incubated in light.
日本植物生理学会の論文