Chromatin subunit structure in different tissues of higher plants

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The basic chromatin structure of higher plants ( Vicia faba and Trillium kamtschaticum) was examined biochemically. After digestion with micrococcal nuclease, the chromatins of these species yielded DNA-protein components which sedimented as discrete peaks at 11S, 15S, 19S, and so on in a sucrose gradient. The buoyant density of Vicia chromatin subunits was about 1.44 g・cm^<-3> in CsCl. Polyacrylamide gel electrophoresis of histone from these subunits of Vicia and Trillium chromatins indicated that the 11S monomer contained very little histone H1 but a full complement of all other histones, whereas the oligomers contained H1 as in the case of undigested chromatin. Therefore, the mode of organization of basic chromatin structure in higher plants is identical with that reported with various other eukaryotes, although two plant histone components are different from the corresponding mammalian histones, H2A and H2B, in molecular weight and amino acid composition. The results indicated also that chromosomes prepared from Trillium meiotic cells do not differ from chromatins of Trillium or Vicia somatic cells in the sensitivity to nuclease digestion or in the size of their subunits.
日本植物生理学会の論文