Preferential inhibition by ethidium bromide of photosynthetic apparatus formation in Rhodopseudomonas spheroides cells

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Ethidium bromide (EB) inhibited growth of cells of the non-sulfur purple photo-synthetic bacterium Rhodopseudomonas spheroides. The inhibitory action of EB on the light-anaerobic cultured cells was stronger than on dark-aerobic cultured ones. EB also suppressed the induced synthesis of bacteriochlorophyll (Bchl) and carotenoids in the dark-aerobically grown cells incubated with gentle aeration under no-growth conditions, suggesting that the target of the inhibitory action of EB on the photosynthetic growth of R. spheroides cells is chromatophore formation. EB depressed the incorporation of ^3H- or ^<14>C-uracil into both RNA and DNA fractions from cells incubated with gentle aeration. In contrast, inhibition by EB of ^3H-uracil incorporation into the DNA fraction was not observed under vigorous aeration. Our findings seemed to favor the hypothesis described previously (10) that lowering the intracellular oxidation-reduction potential might bring about a unique synthesis or turnover of DNA responsible for chromatophore formation.
日本植物生理学会の論文