Improvement of the indolo-α-pyrone fluorescence method for quantitative determination of endogenous indole-3-acetic acid in lettuce seedlings

概要

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The indolo-α-pyrone fluorescence method was modified to measure the endogenous amount of indole-3-acetic acid (IAA) in lettuce seedlings, because impurities in lettuce extracts made the indolo-α-pyrone solution turbid, if the reaction of the extracts with acetic anhydride to give indolo-α-pyrone was disrupted with 3 ml distilled water. This turbid solution caused light scattering, resulting in overestimating the IAA amount in lettuce seedlings. We could, however, obtain a clear indolo-α-pyrone solution by disrupting the reaction with 0.2 ml distilled water and 2.8 ml acetic acid in place of 3 ml distilled water. We examined the reliability of the new method and found that the use of acetic acid substantially slowed down the rate of indolo-α-pyrone breakdown and did not decrease the sensitivity of the method. The effect of gibberellic acid on the endogenous amount of IAA in lettuce seedlings was also examined.
日本植物生理学会の論文