Studies of pesticides effects on Chlorella metabolism III. Effect of isopropyl 3-chrocarbanilate (chlorpropham) on cell cycle and biosynthesis

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Isopropyl 3-chlorocarbanilate (chlorpropham) inhibited Chlorella growth by 50% at 1.3 μM under non-photosynthetic conditions. Average DNA content per cell was increased 2.5-fold by chlorpropham treatment at 4.7 μM. Oxygen uptake was not significantly affected at 47 μM. Protein synthesis was more sensitive to chlorpropham than the other biosynthetic processes tested, but it was not inhibited at 1.3 μM. In cell cycle studies performed under photosynthetic conditions, 50% inhibition of growth occurred at 4 μM. At 14 μM, growth in terms of cell number was completely suppressed while inhibition of growth in terms of average cell volume was partial, resulting in comparatively larger cell volume. This was accompanied by 3.0-fold increase in average DNA content per cell.
日本植物生理学会の論文