Carrier-mediated transport of metabolites in purified bean mitochondria

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概要

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• The mechanism of transport of Krebs cycle intermediates, phosphate and sulfur-containing compounds across the membrane of purified bean mitochondria was investigated by directly measuring the exchange between intramitochondrial labelled substrates and external anions and by testing the inhibitor sensitivity of these transport processes. 1) The exchange between intramitochondrial phosphate and external phosphate or sulfite is insensitive to N-ethylmaleimide or butylmalonate when either is added alone, but is completely inhibited by N-ethylmaleimide plus butylmalonate or by mersalyl. Internal phosphate is exchanged with malate, succinate, oxaloacetate, sulfate and thiosulfate; these reactions are inhibited by butylmalonate but not affected by N-ethylmaleimide. 2) Internal sulfate is exchanged with malate, malonate, succinate, phosphate and sulfite in a butylmalonate- and mersalyl-sensitive reaction. Also the exchanges of malonate with phosphate, sulfate and sulfite are inhibited by butylmalonate and mersalyl. On the other hand, the exchange between intra- and extramitochondrial malonate is completely inhibited only by the combination of butylmalonate and 1,2,3-benzenetricarboxylate. 3) Citrate is exchanged with some di- and tricarboxylates and phosphoenolpyruvate (but not with phosphate, sulfate, oxoglutarate, trans-aconitate and benzenetricarboxylates). These exchanges are inhibited by 1,2,3-benzenetricarboxylate, but not by 1,2,4-benzenetricarboxylate or 1,3,5-pentanetricarboxylate. 4) Oxoglutarate is exchanged with succinate, malate, malonate and oxaloacetate (but not with phosphate, citrate or phosphoenolpyruvate) in a mersalyl-insensitive, butylmalonate- and phenylsuccinate-sensitive reaction. 5) We concluded that bean mitochondria contain the following transport systems: a phosphate carrier inhibited by N-ethylmaleimide or mersalyl, a dicarboxylate carrier inhibited by butylmalonate or mersalyl, a citrate carrier inhibited by 1,2,3-benzenetricarboxylate and an oxoglutarate carrier inhibited by phenylsuccinate or butylmalonate but insensitive to mersalyl.

• 日本植物生理学会の論文

著者

• Desantis Aurelio

  Institute Of Botany University Of Bari

• Palmieri Ferdinando

  Institute Of Biochemistry University Of Bari

• Arrigoni Oreste

  Institute Of Botany University Of Bari

関連論文

• The mechanism of phosphate permeation in purified bean mitochondria
• Carrier-mediated transport of metabolites in purified bean mitochondria

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