Ionic composition and pH of the vacuolar sap in marine dinoflagellate Noctiluca

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Ionic composition of the vacuolar sap of Noctiluca miliaris was as follows: [Na^+] = 487.3 mM, [K^+] =24.1 mM, [Ca^<2+>] =6.6 mM, [Mg^<2+>] =2.8 mM, [Cl^-] =500 mM. [NH_4^+] = 15-25 mM, and [SO_4^<2->] =undetectable. To measure the vacuolar pH of single living cells, a pH-sensitive glass microelectrode was used. The vacuolar pH value was 3.50 ± 0.18. When the cells were transferred from normal sea water into osmotically adjusted 50% sea water for one day, the vacuolar ion concentrations remained almost constant. Upon immersing the cells in osmotically unadjusted sea water of various concentrations for one day, the observed increments or decrements of the vacuolar ion concentrations could be accounted for largely by the migration of water out of or into the cells. The intrinsic ionic composition of the vacuole seems to be constant against changes in ion concentrations of the bathing medium.
日本植物生理学会の論文