Protoplasts as a tool for isolating functional chloroplasts from leaves

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概要

• 論文の詳細を見る

• Leaf protoplasts from various grasses can be used for isolating chloroplasts with high photosynthetic activity. The protoplasts were stable for more than 20 hr during which time chloroplasts could be isolated from protoplasts without any loss of original CO_2 fixation capacity (100-157 μmoles/mg chl・hr). Using Triticum aestivum to optimize assay conditions, the pH optimum for CO_2 fixation by the chloroplasts isolated from protoplasts was between 8.2 and 8.6. Magnesium (0.75 mM) was required for maximum CO_2 fixation by the isolated chloroplasts and sodium ascorbate in the medium allowed a more linear increase in CO_2 fixation with time. Based upon ^<14>CO_2 fixation and ferricyanide-dependent oxygen evolution as criteria of intactness, chloroplasts from protoplasts exhibited a high degree of intactness compared to those obtained by mechanical grinding. Chloroplasts isolated from grass leaves by mechanical grinding had a relatively low capacity for endogenous CO_2 fixation and required addition of ribose-5-phosphate and ADP for maximum activity.

• 日本植物生理学会の論文

著者

• Edwards G.e.

  Horticulture Department University Of Wisconsin

• Rathnam C.K.M.

  Horticulture Department, University of Wisconsin

• Rathnam C.k.m.

  Horticulture Department University Of Wisconsin

関連論文

• Protoplasts as a tool for isolating functional chloroplasts from leaves

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