An electrophoretic analysis of the acid-insoluble chromosomal proteins from different organs of maize seedlings

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Nonhistone chromosomal proteins (NHCP) appear to play an important role in controlling transcription and therefore development; however, these proteins have been studied relatively little in plants. Here the acid-insoluble NHCP from the epicotyls, mesocotyls and roots of 4-5 day-old corn seedlings are compared and fractioned. Nuclei and thence chromatin were prepared as described (23). After removal of the histones by extraction with H_2SO_4, the remaining NHCP were dissolved with sodium dodecyl sulfate (SDS) containing mercaptoethanol and electrophoresed on SDS polyacrylamide gels. On the gels, these acid-insoluble NHCP gave at least 27 bands corresponding to molecular weights from 15,000 to 180,000. Although the mass ratios for acid-insoluble NHCP/DNA differed for different tissues, the electrophoretic patterns were indistinguishable. Subsets of the total NHCP were obtained by fractionations employing sequential extractions at different NaCl concentrations, phenol extractions (2 different procedures) and absorption onto calcium phosphate gel; nevertheless, the electrophoretic patterns of corresponding fractions from different organs looked very similar.
日本植物生理学会の論文