Disruption of certain aleurone grains by various homogenization agents

概要

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Although aleurone grains of certain oilseeds (e.g. peanuts) are exceedingly sensitive to aqueous solutions, it has been reported that aleurone grains maintain their integrity when homogenized in an aqueous solution containing 15% Carbowax 6000 (PEG-6000). Presumably this substance acts as an osmotic agent. We have been unable to confirm this result using microscopic and chemical studies. However, we found that PEG-6000 acts as a protein precipitant; therefore, it is suggested that the pellet obtained upon centrifugation of the homogenate is mostly protein precipitate rather than intact aleurone grains.
日本植物生理学会の論文