An electrophoretic analysis of the acid-soluble chromosomal proteins from different organs of maize seedlings

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Chromatin was prepared by 2 methods from isolated nuclei of the epicotyl, mesocotyl, and root of 4- to 5-day old maize seedlings. Extraction of the nuclei with Tris-EDTA followed by the precipitation of chromatin with 10 mM CaCl_2 isolates approximately 80% of the DNA present in these nuclei. Similarly, the extraction of nuclei with 2 M NaCl isolates a chromatin which can be compared to the CaCl_2-chromatin. Chromatins from all three organs had protein-DNA ratios between 2 and 3, and RNA-DNTA ratios of approximately 0.20 to 0.25. Both chromatin procedures isolate a product which is similar in composition. Acid-soluble proteins of the chromatin were extracted with 0.2 M H_2SO_4 and were analyzed by acid-urea polyacrylamide electrophoresis. The results demonstrated that maize histones consist of 5 major bands which are similar to the bands found in animal systems. Some 11 minor bands were also observed in each preparation from each organ. Both the major and minor bands were identical in preparations from epicotyl, mesocotyl, and root. Acid-soluble proteins prepared from the 2 M NaCl-chromatin were identical to that of the CaCl_2-chromatin except that the minor bands were present in lesser amounts. With the use of a microdensitometer, it was possible to show that the major bands were present in the same relative proportions in each of the seedling organs. A brief survey of chromatins from mature leaves, mature internodes, young tassels, and pollen showed that they contained the same distinct histone bands as above, and no organ-specific histone could be demonstrated.
日本植物生理学会の論文

An electrophoretic analysis of the acid-soluble chromosomal proteins from different organs of maize seedlings

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