Indirect stimulation of protein synthesis by indoleacetic acid in the mung bean hypocotyl

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No exact estimation of the amount of radioactive free amino acids in the cells of the tissue with large size of apparent free space was possible, since the exact size of the apparent free space cannot be measured. Furthermore, estimation of the size of the protein precursor pool, using the method of Holleman and Key, was not possible in hypocotyl sections of mung bean (Phaseolus mungo L. cv. Black), because of the great difference over the length of a section in the rate of the incorporation of leucine-^<14>C into protein. Also, most of the radioactivity in the active pool disappeared within 10 min of the chase period in the presence or absence of IAA, before the effect of IAA on protein synthesis was shown. Thus, neither can the pulse-chase experiment be used to study auxin-induced protein synthesis. IAA stimulated neither the formation of amino acids from acetate-^<14>C, nor the incorporation of the newly formed amino acids into protein. However, IAA did stimulate both the uptake of sucrose-^<14>C and pyruvate-^<14>C into tissue and/or the formation of amino acids from these substances, which resulted in stimulation of the incorporation of these radioactive amino acids into proteins. Enhancement effects of IAA on the rates of amino acid formation and the incorporation of amino acids into protein were of the same magnitude. These results indicate that radioactive amino acids are spontaneously incorporated into proteins without any positive effect by IAA. Furthermore, IAA protects the degradation of some protein fractions. All this evidence raises questions as to the validity of the hypothesis that auxin promotes protein synthesis.
日本植物生理学会の論文

Indirect stimulation of protein synthesis by indoleacetic acid in the mung bean hypocotyl

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