Acid phosphatase in plant tissues I. Changes in activity and multiple forms in tea leaves and tomato fruit during maturation and senescence
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概要
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Multiple forms of acid phosphatase varied in tea leaves (Camellia sinensis L.) depending on variety and the age of the leaves. Polyacrylamide gel electrophoresis indicated the presence of six multiple forms in extracts of young leaves and seven in extracts of mature leaves of the variety Hatzumomizi. Variety Benihomare showed five bands in zymograms of young-leaf extracts and six in zymograms of mature-leaf extracts. In addition to the appearance of the new band, the relative intensity of staining in two other bands changed during maturation. One decreased and one increased in intensity. The components were partially separated by CM-cellulose chromatography and isoelectric focusing. Total activity of acid phosphatase did not change greatly on a fresh-v.'eight basis during maturation and aging. Extracts of immature green tomato (Lycopersicon esculentum Mill.) fruit showed six bands on polyacrylamide gels. Isoelectric focusing and subsequent electrophoresis of the fractions obtained at different pH values, produced evidence for seven acid phosphatase components in tomato extracts. Unlike the situation with certain other climacteric fruit, acid phosphatase in tomato extracts decreased steadily during ripening to a level of activity only 25-40% that of immature green fruit extracts. It is therefore concluded that the onset of senescence in climacteric fruits is not dependent on an increase in acid phosphatase. Substrate specificity, response to inhibitors, K_m, and pH optima of partially purified fractions were similar to those of tea-leaf extracts.
- 日本植物生理学会の論文
著者
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Takeo Tadakazu
Ministry Of Agriculture And Forestry Tea Research Station
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Baker James
United States Department Of Agriculture Ars Agricultural Research Center Agricultural Marketing Rese