The action of cyclic-AMP on GA_3 controlled responses I. Induction of barley endosperm protease and acid phosphatase activity by cyclic-3',5'-adenosine monophosphate

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The effect cyclic-AMP on the induction of protease and acid phosphatase activity in embryoless barley half-seeds was examined. Promotion of protease synthesis was observed with 5 mM cyclic-AMP and ADP. The protease induced by these compounds was similar to protease obtained with GA_3, in that the enzyme was dependent on β-melrcaptoethanol for enhanced activity and increased protease activity observed in the media after a 48 hr incubation with cyclic-AMP and ADP was due to an increase in both the synthesis and release of this enzyme. Of a variety of adenine compounds and nucleosicd diphosphates tested only cyclic-AMP and ADP induced protease activity. When these same compounds were tested for their ability to induce acid phosphatase activity only cyclic-AMP was consistently found to be active. The total amount of enzyme produced as well as the rate of enzyme release was similar for half-seeds incubated with 5 mM cyclic-AMP or a saturating GA_3 concentration. Inhibition of the hormone or nucleotide induced promotion of acid phosphatase was observed when abscisic acid was added to the incubation medium with one of these compounds. Some of the implications of these results are discussed.
日本植物生理学会の論文