Tea leaf peroxidase-Isolation and partial purification

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概要

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• Tea leaf peroxidase occurred as the insoluble from in a homogenate. A large part of the peroxidase from tea leaves was solubilized in a homogenate by adding Tween-80. After fractionation by 0.3 saturation with (NH_4)_2SO_4 and filtration of the homogenate through a Sephadex G-25 column, specific activity of the peroxidase in the effluent increased about 25 times. Crude peroxidase was fractionated into six components; A-1, A-2, B-1, B-2, B-3 and C, by DEAE- and CM-cellulose column chromatography. The fractions showed different mobilities on disc gel electrophoregram at pH 4.2. An isoelectric focusing experiment indicated that pl values of the A-1 and A-2 com-ponents are 5.4 and 5.0, repsectively.

• 日本植物生理学会の論文

著者

• Kato Yoshitake

  Tea Research Station Ministry Of Agriculture And Forestry:(present)national Institute Of Agricultura

• Takeo Tadakazu

  Tea Research Station Ministry Of Agriculture And Forestry

関連論文

• Tea leaf peroxidase-Isolation and partial purification

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