Factors controlling rates of nonphotochemical transformation of Pisum phytochrome in vitro

概要

論文の詳細を見る
Nonphotochemical transformations of the far-red absorbing form of phytochrome, such as its decay or dark reversion to Pr, were studied with solutions obtained from etiolated pea epicotyl tissues at various steps of purification. At pH 7.8, the rate of dark Pfr reversion became significantly faster after the crude extract was purified by gel filtration, but that of well purified solutions was quite low. Decay of Pfr was not seen during any purification step at an alkaline pH, but it occurred in the acidic range of pH even in the presence of sulfhydryl compounds. The rate of Pfr reversion was also influenced by pH; it increased with an increasing pH. Dark reversion of Pisum Pfr was confirmed to proceed in a short, rapid initial phase followed by a slow phase.
日本植物生理学会の論文