Effects of N-1-naphthylphthalamic acid on the growth and bud formation of tobacco callus grown in vitro

概要

論文の詳細を見る
The effect of N-1-naphthylphthalamic acid (NPA), indole-3-acetic acid (IAA) and kinetin on callus growth and bud formation was studied mainly by a tobacco callus culture method. Callus produced from Nicotiana tabacum var. Wisconsin 38 was used as the test plant material. Callus growth on nutrient agar containing 2mg/liter of IAA was promoted by NPA added at a concentration of 0.5mg/liter with 0.4mg/liter of kinetin or by NPA added at 5mg/liter in the absence of kinetin. At a high concentration of 50mg/liter, however, NPA inhibited growth on the medium containing 2mg!liter IAA and no kinetin. Kinetin reduced this NPA inhibition. In the presence of 0.4mg/liter kinetin and 2mg/liter IAA, when the concentration of NPA was 50mg/liter, buds were initiated after calluses were grown on the test medium for 7 weeks in dim light, but no buds formed when NPA was omitted from the above medium. The control of callus growth and bud initiation is based on the active ratio of auxin (IAA) to cytokinin (kinetin) ill the medium and NPA added to the medium can promote or inhibit callus growth and induce bud formation. Therefore, it is proposed that NPA can itself reduce auxin activity or enhance cytokinin activity and hence change the active ratio of the two regulators. NPA may enhance the activity of cytokinin (here supplied as kinetin) but cannot substitute for it.
日本植物生理学会の論文
1970-08-00