Crystallization and some properties of cryptocytochrome c from aerobically-grown Pseudomonas denitrificans

概要

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1. Analyses of cytochrome types in intact cells of aerobically- and anaerobically-grown Ps. denitrificans indicated a higher ratio of cytochrome c to cytochrome b in the former than in the latter. 2. Anaerobically-grown cells contained about two times as much cryptocytochrome c as did aerobically-grown cells. 3. Crystalline cryptocytochrome c obtained from the soluble fraction of cell-free extracts of aerobically-grown cells manifested the same properties as cryptocytochrome c from anaerobically-grown cells, i. e., absorption maxima, autooxidizability, redox potential, molecular weight, haem content, etc. 4. Cryptocytochrome c was reversibly converted to a true haemochrome type spectrum by alcohols, detergents, carboxylic acid salts, guanidine salt or high pH carboxylic acid salts values.
日本植物生理学会の論文