BIOCHEMICAL STUDIES ON COCHLIOBOLUS MIYABEANUS VII. CELLULAR CARBOHYDRATE AS AN ENDOGENOUS SUBSTRATE FOR CONIDIA GERMINATION IN COCHLIOBOLUS MIYABEANUS
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概要
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The carbohydrate content in the conidia of Cochliobolus miyabeanus was found to be about 32% of the dry conidia, approximately 70 per cent of which was composed of an alkali-stable fraction, and the alkali-stable fraction decreased markedly after the germination. The alkali-stable carbohydrate gave, on acid hydrolysis, glucose alone. Starved conidia consumed glucose, maltose, α,β-limit dextrin, and also dextran as exogenous substrates for respiration. On the other hand, germ-tube elongation proved to be stimulated by glucose, maltose, dextran and α,β-limit dextrin as well, but not by soluble starch, glycogen and glutinous rice starch. Accordingly it follows that an alkali-stable carbohydrate including terminal glucopyranose residues combined through α-1,6-glucoside linkage seems to play an essential role in the production of endogenous energy necessary for the germination of conidia in this mold. Moreover, it is noteworthy that dextran was not used as a nutrient for mycerial growth, whereas soluble starch was found to be an efficient carbon source.
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