SYNCHRONOUS CULTURE OF CHLORELLA I. KINETIC ANALYSIS OF THE LIFE CYCLE OF CHLORELLA ELLIPSOIDEA AS AFFECTED BY CHANGES OF TEMPERATURE AND LIGHT INTENSITY
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1. Using the technique of synchronous culture, investigations were made of the effects of temperature and light-intensity on cellular life cycle of Chlorella ellipsoidea. Some improvements in the culture technique for obtaining a good synchrony of algal growth were described. 2. By following the changes of average cell volume and cell number occurring during culturing, the rates of the following processes of life cycle were determined: (i) "growth" (or the increase in cell mass) occurring from the stage of smaller cells (D_a) to the stage of lipened cell (L_3), (ii) "ripening" (or process of formation of " nuclear substances" as estimated from the average number of daughter cells formed from single mother cell), and (iii) "maturing and division" which leads to the full maturation of mother cells (L-cells) and their division into separate daughter cells (D-cells). 3 "Growth" and "ripening" were found to be dependent on both temperature and light intensity, while " maturing and division" were temperature-dependent but light-independent. The average cell volume at the stage of maturing was found to b3e markedly modified by light intensity; larger at higher light intensities. 4. Chenges in incubation temperature ( under the condition of saturating light intensities) were found to affect the life cycle in the following way: (i) The time of onset of "maturing and division" (τ_D), varies markedly with culturing temperature; earlier at higher temperatures. (ii) The average cell volume at this stage also depends on temperature; smaller at higher temperatures. 5. The average number of daughter cells (n) emerging from single mother cells, was found to be uninfluenced by culturing temperature ; (4.0-4.1 under light of saturating intensity) . It was found that the division number n is remarkably varied by changing the light intensity in the "growth" and "ripening" phases; 2.0 at 1 kilolux, 3.7 at 5 kilolux. 4.2 at saturating light intensities (10 and 25 kilolux). This finding was explained by assuming a light-dependent formation of " nuclear substances" during the "growth" and "ripening" phases, the quantity of the substances in the cell at L_3 stage determining the division number. 6. The experimental data were analyzed reaction kinetically, the rate constants and other characteristics of the reactions constituting the processes of life cycle were determined, and values for the apparent activation energy for each reaction were computed. The reactions were discussed with special reference to their relationship with photosynthetic process.
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関連論文
- SYNCHRONOUS CULTURE OF CHLORELLA I. KINETIC ANALYSIS OF THE LIFE CYCLE OF CHLORELLA ELLIPSOIDEA AS AFFECTED BY CHANGES OF TEMPERATURE AND LIGHT INTENSITY
- SYNCHRONOUS CULTURE OF CHLORELLA II. CHANGES IN CONTENT OF VARIOUS VITAMINS DURING THE COURSE OF THE ALGAL LIFE CYCLE