Determination of Lysozyme Chloride in Antiphlogistics by Reversed-Phase High-Performance Liquid Chromatography

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概要

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• An improved reversed-phase high-performance liquid chromatographic method is proposed for the determination of lysozyme. The protein was separated on a short column (4mm i.d.×50mm) packed with octadecylsilane-bonded silica gel (Nucleosil 10C_<18>) by linear gradient elution using 0.1 M sodium phosphate buffer, pH 2.0 (solvent A) and a mixture of isopropanol, ethylene glycol and solvent A (3 : 1 : 1), apparent pH 2.0 (solvent B). Monitoring was done with a ultraviolet detector. Under the elution conditions, lysozyme was recovered from the column in a high yield (97%). This method was found to be applicable to the determination of lysozyme chloride in antiphlogistics.

• 公益社団法人日本薬学会の論文
• 1983-10-25

著者

• 東久世 秀昭

  Tokyo Research Laboratory Shimadzu Corporation

• 早川 江

  (株)島津製作所応用技術部

• 岡田 栄二

  Tokyo Research Laboratory, Shimadzu Corporation

• 川本 健志

  Tokyo Research Laboratory, Shimadzu Corporation

• 川本 健志

  Tokyo Research Laboratory Shimadzu Corporation

• 岡田 栄二

  Tokyo Research Laboratory Shimadzu Corporation

関連論文

• Studies on Bile Acids and Bile Alcohols. II.Separation of Bile Acids by Gas Liquid Chromatography
• Studies on Bile Acids and Bile Alcohols. I. Gas Liquid Chromatography of Human Bile Acids.
• 高速液体クロマトグラフィーによる医薬品中のコンドロイチン硫酸の分析(医薬品の分析)
• Determination of Lysozyme Chloride in Antiphlogistics by Reversed-Phase High-Performance Liquid Chromatography

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