Intracellular Glutathione Levels in Human Colon Cancer Cells Naturally Resistant to Cross-Linking Agents
スポンサーリンク
概要
- 論文の詳細を見る
Correlation between sensitivity to two cross-linking agents, 1-(4-amino-2-methylpyridine-5-yl)-methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU) and cisplatin (DDP), and intracellular glutathione (GSH) level was investigated for two naturally drug-resistant human colon cancer cell lines in comparison with two drug-sensitive human leukemia cell lines. As a result, no appreciable correlation was observed between them. We also studied the possibility that DL-buthionine-s, R-sulfoximine (BSO), an inhibitor of GSH biosynthesis, can sensitize the cancer cells to these anticancer agents via depletion of intracellular GSH. It was found that BSO potentiated ACNU cytotoxicity against human leukemia K562 cells and DDP cytotoxicity against K562 and human colon cancer WiDr cells. It indicates that cancer cells with higher GSH level are more effectively sensitized by BSO regardless of degree of their intrinsic sensitivity to these anticancer agents. These results suggest that intracellular GSH level is not a common mechanism for natural resistance to cross-linking agents in human colon cancer cells but one of the determinants of sensitivity to these anticancer agents of GSH-rich cells.
- 社団法人日本薬学会の論文
著者
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KUBODERA Akiko
Faculty of Pharmaceutical Sciences, Science University of Tokyo
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Ozawa Shogo
Cancer Chemotherapy Center Japanese Foundation For Cancer Research:(present Address)department Of Ph
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Kubodera A
Sci. Univ. Tokyo
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IWATA Kazuyasu
Cancer Chemotherapy Center, Japanese Foundation for Cancer Research
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INABA Makoto
Cancer Chemotherapy Center, Japanese Foundation for Cancer Research
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Inaba Makoto
Cancer Chemotherapy Center Japanese Foundation For Cancer Research
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Iwata Kazuyasu
Cancer Chemotherapy Center Japanese Foundation For Cancer Research:faculty Of Pharmaceutical Science
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Kubodera Akiko
Faculty Of Pharmaceutical Science Science University Of Tokyo
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