Preventive Effects of Zinc on Cadmium-Induced Inhibition of Alkaline Phosphatase Activity and Mineralization Activity in Osteoblast-Like Cells, MC3T3-E1
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概要
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In order to determine the specific action of cadmium on bone metabolism, the effect of cadmium on alkaline phosphatase activity, a marker enzyme of osteoblasts, was compared with that of other divalent heavy metal ions, i.e., zinc, manganese, lead, copper, nickel and mercury (10 μM each), using cloned osteoblast-like cells, MC3T3-E1. Cadmium had the strongest inhibitory effect on alkaline phosphatase activity of the cells among the metals tested. At the same dose, however, cadmium failed to inhibit cellular glucose-6-phosphatase and lactate dehydrogenase activities, suggesting that the inhibitory effect of cadmium on alkaline phosphatase was specific and was not dependent on cell injury. Cadmium treatment caused a significant decrease in cellular zinc level, but mercury treatment had no such effect at the dose inhibiting alkaline phosphatase activity. There was a good correlation between decrease of cellular zinc level and inhibition of alkaline phosphatase activity in cadmium-treated cells. Concomitant treatment of the cells with zinc prevented the cadmium-induced inhibition of alkaline phosphatase activity. However, this was not the case in the mercury-induced inhibition. Cadmium also inhibited the mineralization of osteoblasts. When 10 or 20 μM zinc was concomitantly added to the cultures, the inhibition of mineralization was prevented. These data suggest that the inhibitory effect of cadmium in osteoblasts may be closely related to its influence on the cellular zinc metabolism.
- 公益社団法人日本薬学会の論文
著者
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Yamane Yasuhiro
Faculty Of Pharmaceutical Sciences Chiba University
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Morita Ikuo
Section Of Physiological Chemistry Institute Of Stomatognathic Science Tokyo Medical And Dental Univ
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Yamane Yasuhiro
Faculty Of Pharmaceutical Science Chiba University
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Murota Sei-itsu
Department Of Internal Medicine Tokyo Metropolitan Bokutou General Hospital
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SUZUKI YOSHIHARU
Faculty of Pharmaceutical Science, Chiba University
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MORITA Ikuo
Faculty of Pharmaceutical Science, Chiba University
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MUROTA Seiitsu
Faculty of Pharmaceutical Science, Chiba University
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Suzuki Yoshiharu
Faculty Of Pharmaceutical Science Chiba University
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